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Extra virgin olive oil nanoemulsion attenuated inflammatory response in lipopolysaccharide-induced sepsis
Anna Surgean Veterini1, Nancy Margarita Rehatta1, Subijanto Marto Soedarmo2, Heni Rachmawati3, Widjiati4, Widodo Jatim Pudjirahardjo5, Annis Catur Adi6, I Ketut Sudiana7
1 Department of Anesthesiology and Reanimation, Faculty of Medicine, Universitas Airlangga – Dr. Soetomo General Academic Hospital Surabaya, Bandung, Indonesia
2 Department of Pediatrics, Faculty of Medicine, Universitas Airlangga - Dr Soetomo General Academic Hospital Surabaya, Bandung, Indonesia
3 School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
4 Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
5 Department of Health Administration and Policy, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia
6 Department of Nutrition, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia
7 Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Correspondence Address:
Dr. Anna Surgean Veterini
Department of Anesthesiology and Reanimation, Faculty of Medicine, Universitas Airlangga – Dr. Soetomo General Academic Hospital, Surabaya
Indonesia
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/mtsp.mtsp_11_21
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The provision of nutritional components in critical illness such as sepsis remains a big issue in clinical application, particularly through oral route due to intestinal integrity damaged-associated absorption problem. The aim of this research was to develop Extra Virgin Olive Oil (EVOO) nanoemulsion as a nutrient carrier to improve its permeability while maintaining the intestinal mucosa integrity in mouse model of lipopolysaccharide-induced sepsis. EVOO nanoemulsion was prepared by using ultrasonication-mild agitation method. EVOO nanoemulsion (1.5 mL) was administered to the mice through orogastric tube. The effect of EVOO nanoemulsion was evaluated by assessing the histopathological alterations in lung, measuring the activation of NFκB-p65 by immunohistochemistry of lung tissue, the levels of circulating Surfactant Protein-D (SP-D), tumor necrosis factor-alpha, interleukin (IL)-8, and IL-10. The main result, EVOO nanoemulsion decreased circulating SP-D level after 24 h. In conclusion, EVOO nanoemulsion is a promising carrier to improve nutrition absorption and decrease circulating SP-D as organ injury biomarker.
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