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Table of Contents
ORIGINAL ARTICLE
Year : 2021  |  Volume : 5  |  Issue : 3  |  Page : 60-67

Ecofriendly Alternatives to Antibiotics for Improving Growth Performance in Poultry


1 Department of Animal Nutrition and Feed Science, College of Animal Science, South China Agricultural University, Guangzhou, China
2 Department of Animal Breeding and Biotechnology, Agriculture and Forestry University, Rampur, Chitwan
3 Institute of Agriculture and Animal Science, Tribhuvan University, Bharatpur, Nepal

Date of Submission03-Sep-2020
Date of Acceptance06-Oct-2020
Date of Web Publication1-Feb-2022

Correspondence Address:
Dr. Ishwari Gyawali
Department of Animal Nutrition and Feed Science, South China Agricultural University, Guangzhou
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/MTSP.MTSP_14_20

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  Abstract 


Background: Poultry farming has become an integral part of animal husbandry and has been developed rapidly during the last few decades. Antibiotics have been used and adopted as growth promoters for improving feed efficiency and performance in poultry industry. The prolonged use of antibiotics has led to the development of resistant bacteria and destruction of beneficial bacteria. Search on additives that can replace antibiotics without causing any negative impact in productivity and quality has been progressed. Objective: To review the various growth promoters available alternate to antibiotic, that can perform better in the existing condition according to their genetic potential and keep up health, growth, and performance of poultry. Materials and Methods: Our study relies on the literature analysis; clinical and biological data were collected from different literatures and reports. Result of Study: A brief description of alternatives and their efficaciousness, use, and advantage for enhancing production and safeguarding the health of poultry is presented. Although the beneficial activities of the developed alternative are well demonstrated, their mode of actions is not well defined. Conclusions and Recommendations: The article is useful to the researchers to enhance their idea on poultry birds and perform further research on antibiotics and other alternatives without sacrificing birds.

Keywords: Alternatives, antibiotics, applications, poultry


How to cite this article:
Gyawali I, Paudel R, Rayamajhi K, Khan IA, Dahal G. Ecofriendly Alternatives to Antibiotics for Improving Growth Performance in Poultry. Matrix Sci Pharma 2021;5:60-7

How to cite this URL:
Gyawali I, Paudel R, Rayamajhi K, Khan IA, Dahal G. Ecofriendly Alternatives to Antibiotics for Improving Growth Performance in Poultry. Matrix Sci Pharma [serial online] 2021 [cited 2022 Sep 25];5:60-7. Available from: https://www.matrixscipharma.org/text.asp?2021/5/3/60/337085




  Introduction Top


Over more than six decades, dietary antibodies are used not solely to regulate infectious disease but conjointly to improve growth performance and feed potency.[1] They are accountable for building the immunocompetence of poultry against several infectious diseases. However, the use of antibiotics as feed additives, in the long run, can lead to the development of bacteria resistant to drugs that are used to treat infections. Later on, they are of potential risk if they are transferred to humans.[2] In-feed antibiotic (IFA) has played a substantial role in the advancement and prosperity of the poultry industry since its discovery.[3] When antibiotic is administrated, it impacts the entire population of the body. Among the bacteria, some are susceptible to the antibiotics that die, but some bacteria may thrive, and they begin to multiply. Sometimes, these remaining bacteria are resistant to many antibiotics, and many large problems can develop.[4] There exist significant concerns about the use of an IFA, which leads to the development of antimicrobial resistance, creating a potential menace to human health.[5] [Figure 1] shows the effect of antibiotics on different aspect of environment. Due to the negativity and debate on the role of IFA use, the European Union banned the approval of antibiotics as growth promoters since January 1, 2006, on preventive grounds.[6] The reduction in the use of antibiotic growth promoters (AGPs) in the future seems inevitable, and the practice of using antimicrobials may prove economically illogical because of market limitations and export restrictions.[7] The number of scientific papers, researches, and analyses has been increasing, which is concerned with growth promoter and feed additives to push growth, enhance gut health, and reduce the utilization of antibiotics in animal production. Several antibiotic alternatives are available, which are used to increase productivity and create a suitable environment for animals to perform higher in keeping with their genetic potential under commercial conditions. The additives may include probiotics, prebiotics, organic acids, synbiotics, phytogenics, enzymes, antimicrobial peptides, hyperimmune egg antibodies, bacteriophages, clay, and metals [Figure 2].[1] The target of this review is to search out the other alternatives for antibiotics for better growth performance and to extend the feed potency in poultry.
Figure 1: Antibiotics resistance and its effect on different aspects of environment

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Figure 2: Various types of alternatives to antibiotics in poultry production

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  Methodology Top


Our study was based on the literature and report analysis. Precise study was done in line with the topic of the study; relevant information and clinical and biological data related to antibiotics and different growth promoter were collected from journals available on Web of Science, PubMed, and other related sites. Reports from the World Health Organization (WHO), North American Meat Institution, and other organizations were studied to understand the role of food additives, antibiotics, and their impact on health of both bird and human.

Characteristics of antibiotic growth promoter alternatives

An alternative to growth promoter ought to have beneficial features as AGPs. However, the explicit beneficial action of the AGP is not well understood how it works. The most accepted mechanism is that the AGPs have an antibacterial activity that supports the performance in numerous ways: it reduces the incidence and severity of subclinical infections, reduces the microbial uses of nutrient, improves the absorption of nutrient due to the thinning of the intestinal wall, and lowers the amount of growth-depressing metabolites produced by the Gram-positive bacteria.[8] Numbers of scientists have postulated the mechanism of its action at different times. No matter the mechanism, the most important thing is that the selected alternative must improve the performance at least as AGPs. There are many possible ways that microbiota-modulating compounds could alter the intestinal microbiota population without adding AGPs to the feed. The most apparent method is therapeutic doses of antibiotics under prescription, a practice that will undoubtedly increase and possibly raise the probability of the emergence of resistant human pathogens. For the complete loss of AGPs, none of the nonantibiotic AGP alternatives are likely to remunerate. It must be emphasized that some strategies will only help compensate partially but will not replace AGPs and will work through an indirect mechanism.[9]

Classes of alternatives

Several antibiotic alternatives have been proposed and demonstrated in poultry production, including probiotics, prebiotics, synbiotics, organic acids, enzymes, and phytogenics. Newly developed modern alternatives such as bacteriophages, antimicrobial peptides, hyperimmune egg yolk, AMP, and clay have come into practice in recent years.

Probiotics

Probiotics are microbial feed supplements that have a contrasting mode of action, and it improves the microbiota balance in the intestine, inhibiting the growth of pathogenic bacteria, promoting digestion, lowering cholesterol level, and boosting the immune system.[10] There are several sources of probiotics such as live bacteria (Bacillus subtilis, Lactobacillus, Bifidobacterium, and Streptococcus), yeast (Saccharomyces cerevisiae, Saccharomyces boulardii, and Candida), and fungi (Aspergillus). Probiotics can follow various mechanisms based on inhibition of all pathogens via producing organic acids and antibacterial substances, i.e., hydrogen peroxide, bacteriocins, and defenses, blockading of pathogenic bacteria adhesion to intestinal epithelial binding sites using competitive inhibition.[11] The majority of the conducted research aims to investigate the effects of probiotics in reducing the number of pathogenic microorganisms in the gastrointestinal (GI) tract. A single strain of Lactobacillus sp. (Lactobacillus casei, Lactobacillus fermentum, Lactobacillus bulgaricus, Lactobacillus. reuteri), when given with diet, had shown to improve body weight and feed efficiency in broilers.[12] Such type of results was also shown when broilers got multiple strains of Lactobacillus sp.[13] The application of several other bacteria such as Enterococcus faecium, Clostridium butyricum, and Rhodopseudomonas palustris also significantly increased the daily weight gains with decreased feed conversion ratio (FCR).[1] Lei et al. in 2013 reported that the inclusion of Bifidobacterium licheniformis improved laying performance and egg mass.[14] Lactic acid bacteria, when supplemented to broilers' feed, the cholesterol content of meat, were significantly reduced.[15] The addition of probiotics with diet also reduced the number of gut pathogens such as Salmonella enteritidis, Salmonella gallinarum, Salmonella typhimurium, and Campylobacter jejuni.[16] When laying hens were given a diet supplemented with different strains of probiotics, it significantly improved gut microbial balance, blood and yolk cholesterol levels, egg production, and egg quality. Lactobacillus improved the equilibrium of gut microbiota by increasing the population of Bifidobacteria and decreased potentially harmful bacteria.[17] Not all strains exhibit all of the above properties; hence, due care must be taken to select the strains and their combinations to achieve maximum beneficial effect in vivo in poultry. It can be concluded that probiotics can be a potential alternative to antibiotics for increasing poultry growth and performance.

Prebiotics

Prebiotics are those macromolecules that are derived from plants or synthesized by microorganisms. They are fermentable feed additives that can support (directly or indirectly) a healthy intestinal microbiota. They have a beneficial action on GI microbiota and also promote the growth of animals.[18] Mechanisms by which probiotics regulate the ecosystem of the gut include improvement of the epithelium, alternation of the intestinal microbiota, and stimulation of the immune system.[19] The functions of prebiotics include alteration of GI microflora, immune stimulation, prevention colon cancer and reduction of pathogen invasion, reduction of cholesterol and odor compounds, improve gut health through intestinal microbial balance, reduction in ammonia and phenol products promotion of enzyme reaction, and ultimately reduction production cost.[17] Prebiotics include a variety of nonstarch polysaccharides (NSPs) or oligosaccharides, fructooligosaccharides (FOSs), Mannan oligosaccharides (MOSs), oligofructose, inulin, galactooligosaccharides, lactulose, maltooligosaccharides, isomaltooligosaccharide (IOS), lactitol, xylooligosaccharide, glucooligosaccharide, soya-oligosaccharide, and pyrodextrins.[1] The significant characteristics of being good prebiotics include: it should neither be hydrolyzed nor be absorbed in the upper part of the GI tract, and it is easy to process in a larger scale too and induces systemic effect to enhance the health of the host and palatable as feed ingredient.[20]

The addition of various levels of MOS to the broilers diet significantly improved feed conversion efficiency along with intestinal villi height and increased body weight.[21] Specific benefits of the prebiotics were found in reducing blood cholesterol and increasing lactic acid-producing bacteria in broilers.[10] It is reported that when broilers were supplemented with prebiotics, it improved body weight by 5.41%, decreased FCR by 2.54%, and reduced mortality rate by 10.5%.[22] Prebiotics, when used in the layers, improved feed intake, body weight gain, egg production from 20 to 36 weeks of age, FCR, egg weight, egg mass, and egg size from 20 to 52 weeks of age.[23] It is found from various studies that prebiotic supplementation improves meat quality of the produced broilers and egg quality of layers.

Synbiotics

Prebiotics and probiotics serve as an essential product for the development of healthy intestinal microflora. Prebiotics and probiotics act through different mechanisms and in separate compartments of the intestine. Therefore, they can be combined into one synergistic compound called synbiotics.[24] Synbiotics are used as an useful feed additive to improve the meat quality, productive performance, and ammonia reduction and also to decrease the microbial population of broiler chicks.[25] Supplementation of diet with synbiotics showed a significant increase in body weight, average daily gain, feed efficiency, and carcass yield percentage compared to control or probiotics only.[26] Another study showed that a combination of yeast-derived carbohydrates and probiotics increased body weight gain faster than the controls or prebiotics supplements in pullet.[27] Feeding of the symbiotic named Biomin ®IMBO at 0.1%, 0.15%, and 0.125% of the diet to broilers improved body weight gain and feed efficiency in the starter period and have no detrimental effect on their performance.[28] The addition of GOS and B. subtilis to broiler diet showed that it improves the ADG and FCR and also reduces the incidence of diarrhea and mortality. Synbiotic containing a combination of E. faecium and prebiotics derived from chicory and sea algae significantly improved a live weight, ADG, carcass yield, and also FCR. By the combination use of probiotics and prebiotics, it could represent a synergistic strategy to improve poultry intestinal health and also reduce the spread of pathogens in the environment.[16] The use of synbiotics has gained considerable attention in the poultry industry as it can promote healthy intestinal function, improve performance, and keep pathogens in check. The number of research is still inadequate regarding the issue of their working mechanism. If further research is progressed, it can play a pivotal role in poultry to act as an alternative to antibiotics.

Organic acids

Organic acids are either simple monocarboxylic acid such as formic, acetic, propionic, and butyric acid or carboxylic acid having a hydroxyl group such as lactic, malic, tartaric, and citric acid.[29] They are added in the feed or drinking water and can be used either individually as an organic acid or as their salt (sodium, potassium, or calcium) or as blends of multiple acids or their salt.[9] They show their antibacterial nature by decreasing the pH of drinking water and reducing the buffering capacity of the feed that affect the physiology of the crop and proventriculus, which can improve the quality of the egg.[30] Organic acid also ameliorates the quality of the egg, i.e., yolk index, albumen index, shell hardness, and resistance to breakage.[31] The number of researchers delineates the numbers of mechanisms, but it is not clearly understood.

Organic acid alters the gut microflora either by directly killing or through cell wall penetration or indirectly modifies the pH and reduces pathogenic bacteria. At the same time, it increases the acid-tolerant beneficial species such as Lactobacillus spp. so that it reduces the competition for nutrients by altering microbes.[32] It is found that organic acid increases nutrient digestibility by increasing protein and dry matter retention and improving mineral absorption and phosphorous utilization.[33] Several trials and researches have shown that a diet containing organic acid has significantly increased the villus height and area in the duodenum, jejunum, and ileum of chicks.[34] Supplementation of fumaric acid to broiler chicken had shown to improve weight gain and feed efficiency.[35] A similar result was found by using other organic acids such as citric acid and acetic acid in further researches and findings. At the same time, research showed that supplementation of organic acid as the blend was found to be more beneficial than a single acid. Various organic acid blends were tested and found to improve the FCR in broiler chickens.[36] The beneficial effect of organic acid can be summed as it significantly increases villus width, height, and area of the duodenum, jejunum, and ileum of broilers, thus boosting the performance of broilers. It improves the digestibility by reducing the microbial competition with the host for nutrients. They lower the incidence of subclinical infection by secretion of immune mediators, reducing the promotion of ammonia and other growth-depressing microbial metabolites.

Phytobiotics or botanical supplements

Plant extracts are complex compounds containing the different compositions of many active components. Generally, they contain protein, peptides, oligosaccharides, fatty acid, vitamins, and minerals.[37] Many plants and their extract have been used and reported to possess beneficial multifunctional properties due to which it is used as feed additives in the farm from ancient cultures.[9] Plant-derived products are natural, less toxic than antibodies and typically residue-free. Phytobiotics has established a positive effect in the animlas due to the presence of different plant constituents like:glycosides, terpenoids (monoterpenes and sesquiterpenes, steroids), phenolics (tannins), alkaloids (present as alcohols, aldehydes, ketones, esters, ethers, and lactones), flavonoids, and glucosinolate.[38] The coherent mechanism of its mechanism is still not understood, But their features are responsible for the disruption of the cellular membrane of pathogens, protection of intestinal mucosa from bacterial pathogens colonization, promoting the growth of beneficial bacteria such as Lactobacilli and Bifidobacteria, stimulation of immune system especially activation of lymphocytes, macrophages, and NK cells.[39] A wide variety of herbs and spices such as oregano, rosemary, marjoram, garlic, ginger, thyme, turmeric, green tea, black cumin, and coriander have been used in poultry as an AGP alternative. When broilers were given diets supplemented with a mixture of 14 herbs, they showed a significant increase in body weight gain and improvement in feed efficiency.[40] There are several classes of plant products based on physical characters and appearance, which include essential oil, crude or processed plant parts, processed extracts, mixtures of powders or extracts, and phytochemicals used for the prevention and treatment of various diseases in farm animals.[41] Extract from black pepper, cinnamon, and turmeric improves the immune system and reduces serum cholesterol and liver enzymes as well as enhances performance and overall health status of poultry.[37] Botanical or herbal extracts, flavors, and essential oils (EOs) are beneficial in the poultry farm. Use of asthma plant (Euphorbia hirta) increased villus height, crypt depth, and the ratio of villi to the crypt and enhanced the maintenance and functions of the small intestine.[42] Use of 240 ppm dose of ginger rhizome powder enhanced the nutrient digestion and absorption in poultry because of its positive effect in gastric secretion and enterokinase. The experiment done by the use of an extract of Babylon willow (Salix babylonica)[43] and black poplar (Populus nigra)[44] showed that they improve heat tolerance, weight gain, and FCR in poultry. Along with extract and powder, EOs (volatile lipophilic substances obtained by cold extraction or by steam or alcohol distillation)[45] from clove, coriander, star anise, ginger, garlic, rosemary, turmeric, basil, caraway, lemon, and sage have been used either individually or as blends for the betterment of health and performance. The use of EO of garlic (Allium sativum) improved the growth performance and essential microbial population.[46] Another study shows that the EOs of rosemary (Rosmarinus officinalis) and laurel (Laurus nobilis) improve the gut health and act as an antioxidant,[47] and EO supplementation was also shown to improve feed efficiency as seen by reduced FCR. A meta-analysis study of broiler chicken by the inclusion of a commercial blend of phytonutrients containing carvacrol, cinnamaldehyde, and capsicum oleoresin, in feed, increased body weight gain and decreased FCR and mortality.[48] Herbs, spices, and various other plants extracts, including EO, are used as alternatives to antibiotics, while some do have growth-promoting effects, antimicrobial properties, and other health-related benefits.[38]

Antimicrobial peptides

Antimicrobial peptides (AMPs) are the small biological components having a broad-spectrum effect against bacteria, fungi, protozoa, and some of the viruses.[49] Due to the broad-spectrum activity of AMPs, they can be developed as one of the better alternatives to antibiotics.[50] Most AMPs can interact with bacterial membranes. They decrease the pathogen count[51] and also increase the beneficial bacteria, nutrient absorption, weight gain, and FCR.[52] It is generally hypothesized that three main mechanisms could account for peptide permeation of the membrane of the target cell. First, they are responsible for the disruption of DNA, transcription, and translation. They also alter membrane permeability, which leads to cell lysis by the formation of the transmembrane pore. The introduction of AMPs also inhibits cell cycle, activates lytic enzymes, and produces free radicals. Due to this, the production of cytokines occurs by the proliferation of immune cells and they also induce wound repair mechanisms.[53] Along with directly attacking microbes, they also accord protection by the alternative mechanisms such as maintenance of normal gut homeostasis and modulation of host inflammatory response.[49] The application of AMPs has been mostly focused on the pathogens causing infectious diseases rather than growth-promoting activities. Several types of researches have been conducted on its beneficial effect on growth performance, gut microbiology, and intentional morphology. Supplementation of cecropin A(1-11)-D(12-37)-Asn (CADN) in poultry diets increased weight gain, feed intake, and intestinal villus height.[54] Naturally synthesized AMPs, from swine gut and rabbit, administrated in birds improved growth performance, intestinal ability to absorb nutrients, and mucosal immune parameters.[54],[55] Based on the origins of AMPs, there is a particular group of AMPs called bacteriocins; these are commonly described as small ribosomally synthesized peptides that are secreted by bacteria and inhibit the growth of closely related species and found to be potential food additives.[56] Bacteriocins, produced by Ruminococcus albus 7 called Albusin B, were supplemented to poultry feed and showed improved growth performance, increased intestinal absorption, and Lactobacillus counts, and modulated lipid metabolism.[52]

Researchers have concluded that antimicrobial peptides, along with bacteriocins, improve growth performance, gut health, and immune function, and promote nutrient digestibility in poultry. The beneficial effect is due to their antimicrobial and immunomodulating activity. Their potential can be improved further if the number of obstacles such as high production cost, resistance development, and instability of the AMPs is addressed in the future.

Bacteriophage

Bacteriophages are highly species-specific viruses that kill bacteria by the producing endolysins and the subsequent lysis of the bacterial cells. Bacteriophages can be considered safe antibiotic alternatives as they exhibit no activity against animal and plant cells.[57] Phage therapy exploits the therapeutic potential of lytic phage. Bacteriophage binds to the specific receptors on the bacterial cell surface, releases their genetic material into the cell, and uses the host cell machinery to synthesize multiple virion particles. When the virus is matured, the cell wall is lysed and finally releases progeny phages.[58] Not only the whole phages but also phage-encoded enzymes have been found to have antibacterial properties. They are classified into peptidoglycan hydrolases (VAPGH) and endolysins. VAPGH disturbs the peptidoglycan layer of the bacterial cell wall after phage absorption. They produce a small hole in the cell wall to facilitate the transfer of viral DNA into the cytoplasm.[59] Endolysins have a cell wall binding domain which cleaves the specific bonds in the peptidoglycan.[60] Incorporation of 0.035% or 0.05% of bacteriophage in the feed of laying hens significantly improved egg production.[61] It is also reported that supplementation of 0.1% and 0.15% of bacteriophage increased body weight gain and also reduced the FCR.[62] The number of pathogens (Campylobacter spp.) was reduced in broilers when phage was administrated by mixing with drinking water.[63]

Limited number of research has been carried out under the use of bacteriophage as an alternative to antibiotics, and some drawbacks have been found by the use of phage. The development of bacterial resistance is one of the major issues. Numbers of mechanisms have been postulated to discuss this issue such as blocking of viral adsorption on surface receptors, degradation of viral genome by restriction–modification systems, and phage superinfection exclusion, but this can be prevented by using a bacteriophage “cocktail,”[53] but the resistance to bacteriophage is not considered as a serious threat as the number of bacteriophages is specific for the host is not limited.[64] Furthermore, research is needed to establish the performance effects of bacteriophages and make their use practical in poultry production systems.

Antibody

Supplementation of antibodies orally is an upcoming approach for the treatment of pathogens in humans as well as in animals.[53] In the case of poultry, hyperimmune egg yolk antibodies (IgYs) are essential antibody as an alternative. IgYs are produced by repeated immunization of hens with specific antigens, and collection of antibodies, thereafter from their egg yolks, has been commonly employed in the prevention and treatment of various enteric diseases and can be utilized as a feed additive.[65] Mainly, two mechanisms of action have been described as the action of the antibody. First, IgY binds to the bacterial structure such as flagella and pili which prevent the adhesion to and colonization of intestinal epithelium by the bacteria. Second,on binding there is increase on pathogen agglutinates, that lead to changes in structure on cell surface which lead to increase phagocytic activity and toxin neutralization.[66] On the 1990s, progeny from hens injected with jack bean urease improved body weight at 3 weeks of age and was proposed that urease antibodies maternally transferred to the progeny decreased ammonia production in the intestinal tract by inhibiting bacterial urease enzyme and improving growth.[1]

Several studies have reported the efficacy of IgY against E. coli, Clostridium, Campylobacter, and Salmonella.[67] Supplementation of diet with egg powder containing CCK antibodies at 0.25 g/kg increased the feed conversion efficiency as compared to that of birds fed with egg powder from unimmunized hens. Weight gain and FCR at 3 weeks of age were increased by 9% and 8%, respectively, using the yolk antibody.[68] Oral administration of IgY against E. coli O78:K80 via egg yolk powder found to improve intestinal health and broiler's performance.[69] Other many IgYs are used in poultry to improve feed efficiency and as an excellent alternative to antibiotics. Large amount of antibodies can be produced in laying hens via eggs and can be collected noninvasively. They are environment-friendly and less toxic. Although bacteria cannot develop resistance to antibodies, their most significant drawback is proteolytic degradation in the gut as well as their expensive in large-scale production.[65] Although the existing results seemed encouraging and more advantageous, much more research is needed on using egg antibodies for growth promotion by making it cost-effective method for IgY production for higher production in poultry.


  Conclusion Top


Probiotics, prebiotics, and synbiotics interact directly with host gut epithelium, and pathogen colonization is prevented. Organic acid decreases the pH of water intake and has a successive effect on the physiology of the crop and proventriculus by reducing the buffering capacity of feed. Phytobiotics show antioxidant and antimicrobial activity,but the mechanism of its action is not clearly understood, so further study is necessary. AMP is a broad spectrum in nature and binds the cell surface by electrostatic attraction rather than to a specific receptor. A limited number of researches have been carried out in the case of antibody and bacteriophage. There is a great need for the development of antibiotic alternatives that can improve performance and maintain the optimum health of animals. They are used to replace AGPs whose primary function is to decrease the microbial population and promote the growth by a different mode of action, which includes exclusion and inhibition of pathogens in the intestinal tract, improvement of gut integrity, improvement in digestion, and absorption of the nutrient. A variety of alternatives have been used as antibiotics in the poultry industry. Many research results indicated that especially prebiotics, probiotics, synbiotics, organic acids, and phytobiotics showed similar effects to antibiotics in poultry. Besides, appropriate doses and the method of application for these alternatives to antibiotics are important for them to be more effective in poultry. During the selection of alternatives, well care should be taken in such a way that it fits the need of the individual production without affecting the welfare of the poultry. Further research is needed about the mechanism of action and the means to standardize the effects. Combined use of two alternatives with proper management, dose and delivery method, can be practiced for reducing the use of antibiotics and improving the performance of animals. Due to the development of resistance in bacteria by the use of antibiotics, it is essential to develop alternatives with multiple targets for antibiotic activity so that it is hard to develop an effective resistance mechanism against it. The elimination of antibiotics may harm the production, so advanced research is essential to provide new opportunities for developing alternatives to ameliorate the production and health of poultry. During the development, the selection of multiple products that can wok synergetically and act on numerous pathogens simultaneously is required. The number of scientific studies under field conditions on the target animal species is needed to determine the efficacy and safety of alternative products. In course of studies' integration of nutrition, health and disease are vital. The linkage between researchers, feed industry, pharmaceutical industries, poultry industry and regulatory agencies is essential so that it can define the scope of future research, development, and applications for alternatives products to antibiotics.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Gadde U, Kim WH, Oh ST, Lillehoj HS. Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: A review. Anim Health Res Rev 2017;18:26-45.  Back to cited text no. 1
    
2.
Al-Khalaifah HS. Benefits of probiotics and/or prebiotics for antibiotic-reduced poultry. Poult Sci 2018;97:3807-15.  Back to cited text no. 2
    
3.
Gadde UD, Oh S, Lillehoj HS, Lillehoj EP. Antibiotic growth promoters virginiamycin and bacitracin methylene disalicylate alter the chicken intestinal metabolome. Sci Rep 2018;8:3592.  Back to cited text no. 3
    
4.
North American Meat Institution. The Facts about Antibiotics in Livestock & Poultry Production; 2013. Available from: https:// www.meatinstitute.org/index.php?ht=a/GetDocumentAction/ i/99943%0Awww.meatami.com. [Last accessed on 2020 sep 02].  Back to cited text no. 4
    
5.
WHO. The Evolving Threat of Antimicrobial Resistance: Options for Action. WHO Publications; 2012. p. 1-119. Available from: http://www.ijmr.org.in/article.asp?issn=09715916; year=2014;volume=139;issue=1;spage=182;epage=183;aulast=Kapi [Last accessed on 2020 Aug19].  Back to cited text no. 5
    
6.
Castanon JI. History of the use of antibiotic as growth promoters in European poultry feeds. Poult Sci 2007;86:2466-71.  Back to cited text no. 6
    
7.
Hassan HM, Mohamed MA, Youssef AW, Hassan ER. Effect of using organic acids to substitute antibiotic growth promoters on performance and intestinal microflora of broilers. Asian Australas J Anim Sci 2010;23:1348-53.  Back to cited text no. 7
    
8.
Brennan J, Skinner J, Barnum DA, Wilson J. The efficacy of bacitracin methylene disalicylate when fed in combination with narasin in the management of necrotic enteritis in broiler chickens. Poult Sci 2003;82:360-3.  Back to cited text no. 8
    
9.
Huyghebaert G, Ducatelle R, Van Immerseel F. An update on alternatives to antimicrobial growth promoters for broilers. Vet J 2011;187:182-8.  Back to cited text no. 9
    
10.
Tayeri V, Seidavi A, Asadpour L, Phillips CJ. A comparison of the effects of antibiotics, probiotics, synbiotics and prebiotics on the performance and carcass characteristics of broilers. Vet Res Commun 2018;42:195-207.  Back to cited text no. 10
    
11.
Tiwari G, Tiwari R, Pandey S, Pandey P. Promising future of probiotics for human health: Current scenario. Chron Young Sci 2012;3:17.  Back to cited text no. 11
  [Full text]  
12.
Salim HM, Kang HK, Akter N, Kim DW, Kim JH, Kim MJ, et al. Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. Poult Sci 2013;92:2084-90.  Back to cited text no. 12
    
13.
Mookiah S, Sieo CC, Ramasamy K, Abdullah N, Ho YW. Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agric 2014;94:341-8.  Back to cited text no. 13
    
14.
Lei K, Li YL, Yu DY, Rajput IR, Li WF. Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function of laying hens. Poult Sci 2013;92:2389-95.  Back to cited text no. 14
    
15.
Popova T. Effect of probiotics in poultry for improving meat quality. Curr Opin Food Sci 2017;14:72-7.  Back to cited text no. 15
    
16.
Alfred Blanch CH. Role of probiotics, probiotics and synbiotics in poultry nutrition. Int Poult Prod 2016;24:23-4.  Back to cited text no. 16
    
17.
Denli M, Demirel R. Replacement of antibiotics in poultry diets. CAB rev perspectives in agriculture, veterinary science. Nutrit Natl Res 2018;13. [Doi: 10.1079/PAVSNNR201813035].  Back to cited text no. 17
    
18.
Micciche AC, Foley SL, Pavlidis HO, McIntyre DR, Ricke SC. A review of prebiotics against Salmonella in poultry: Current and future potential for microbiome research applications. Front Vet Sci 2018;5:191.  Back to cited text no. 18
    
19.
Teng PY, Kim WK. Review: Roles of prebiotics in intestinal ecosystem of broilers. Front Vet Sci 2018;5:245.  Back to cited text no. 19
    
20.
Yadav AS, Kolluri G, Gopi M, Karthik K, Malik YS, Dhama K. Exploring alternatives to antibiotics as health promoting agents in poultry- A reviewJ Exp Biol Agric Sci 2016;4(Suppl 3):368-83.  Back to cited text no. 20
    
21.
Yang P, Seib PA. Low-input wet-milling of grain-sorghum for readily accessible starch and animal feed. Cereal Chem 1995;72:498-503.  Back to cited text no. 21
    
22.
Hooge D, Connolly A. Meta-analysis summary of broiler chicken. Int J Poult Sci 201;12:1-8.  Back to cited text no. 22
    
23.
Tang SG, Sieo CC, Ramasamy K, Saad WZ, Wong HK, Ho YW. Performance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with prebiotic, probiotic and synbiotic. BMC Vet Res 2017;13:248.  Back to cited text no. 23
    
24.
Slawinska A, Plowiec A, Siwek M, Jaroszewski M, Bednarczyk M. Long-term transcriptomic effects of prebiotics and synbiotics delivered in ovo in broiler chickens. PLoS One 2016;11:1-21.  Back to cited text no. 24
    
25.
Abdel-Moneim AE, Elbaz AM, Khidr RE, Badri FB. Effect of in ovo inoculation of Bifidobacterium spp. on growth performance, thyroid activity, ileum histomorphometry, and microbial enumeration of broilers. Probiotics Antimicrob Proteins 2020;12:873-82.  Back to cited text no. 25
    
26.
Awad WA, Ghareeb K, Abdel-Raheem S, Böhm J. Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult Sci 2009;88:49-56.  Back to cited text no. 26
    
27.
Yitbarek A, Echeverry H, Munyaka P, Rodriguez-Lecompte JC. Innate immune response of pullets fed diets supplemented with prebiotics and synbiotics. Poult Sci 2015;94:1802-11.  Back to cited text no. 27
    
28.
Mousavi SM, Seidavi A, Dadashbeiki M, Kilonzo-Nthenge A, Nahashon SN, Laudadio V, et al. Effect of a synbiotic (Biomin®IMBO) on growth performance traits of broiler chickens. Eur Poult Sci 2015;79:1-15.  Back to cited text no. 28
    
29.
Haq Z, Rastogi A, Sharma RK, Khan N. Advances in role of organic acids in poultry nutrition: A review. J Nat Appl Sci 2017;9:2152-7.  Back to cited text no. 29
    
30.
Van Immerseel F, Russell JB, Flythe MD, Gantois I, Timbermont L, Pasmans F, et al. The use of organic acids to combat Salmonella in poultry: A mechanistic explanation of the efficacy. Avian Pathol 2006;35:182-8.  Back to cited text no. 30
    
31.
Yesilbag D, Çolpan I. Effects of organic acid supplemented diets on growth performance, egg production and quality and on serum parameters in laying hens. Rev Med Vet (Toulouse) 2006;157:280-4.  Back to cited text no. 31
    
32.
Biggs P, Parsons CM. The effects of several organic acids on growth performance, nutrient digestibilities, and cecal microbial populations in young chicks. Poult Sci 2008;87:2581-9.  Back to cited text no. 32
    
33.
Rafacz-Livingston KA, Parsons CM, Jungk RA. The effects of various organic acids on phytate phosphorus utilization in chicks. Poult Sci 2005;84:1356-62.  Back to cited text no. 33
    
34.
Rodríguez-Lecompte JC, Yitbarek A, Brady J, Sharif S, Cavanagh MD, Crow G, et al. The effect of microbial-nutrient interaction on the immune system of young chicks after early probiotic and organic acid administration. J Anim Sci 2012;90:2246-54.  Back to cited text no. 34
    
35.
Banday MT, Adil S, Khan AA, Untoo M. A study on efficacy of fumaric acid supplementation in diet of broiler chicken. Int J Poult Sci 2015;14:589-94.  Back to cited text no. 35
    
36.
Samanta S, Haldar S, Ghosh TK. Comparative efficacy of an organic acid blend and bacitracin methylene disalicylate as growth promoters in broiler chickens: Effects on performance, gut histology, and small intestinal milieu. Vet Med Int 2010;2010:645150.  Back to cited text no. 36
    
37.
Tajodini M, Saeedi HR, Moghbeli P. Use of black pepper, cinnamon and turmeric as feed additives in the poultry industry. Worlds Poult Sci J 2015;71:175-83.  Back to cited text no. 37
    
38.
Diaz-Sanchez S, D'Souza D, Biswas D, Hanning I. Botanical alternatives to antibiotics for use in organic poultry production. Poult Sci 2015;94:1419-30.  Back to cited text no. 38
    
39.
Vidanarachchi JK, Mikkelsen LL, Sims I, Iji PA, Choct M. 2005, Phytobiotics: Alternatives to antibiotic growth promoters in monogastric animal feeds. Rec Adv Anim Nutr Aust 2005;15(Kamel 2001):131-44.  Back to cited text no. 39
    
40.
Guo FC, Kwakkel RP, Soede J, Williams BA, Verstegen MW. Effect of a Chinese herb medicine formulation, as an alternative for antibiotics, on performance of broilers. Br Poult Sci 2004;45:793-7.  Back to cited text no. 40
    
41.
Dhama K, Latheef SK, Mani S, Samad HA, Karthik K, Tiwari R, et al. 2015, Multiple beneficial applications and modes of action of herbs in poultry health and production-A review. Int J Pharmacol 2015;11:152-76.  Back to cited text no. 41
    
42.
Kumar S, Malhotra R, Kumar D. Euphorbia hirta: Its chemistry, traditional and medicinal uses, and pharmacological activities. Pharmacogn Rev 2010;4:58-61.  Back to cited text no. 42
    
43.
Salem AZ, Salem MZ, Gonzalez-Ronquillo M, Camacho LM, Cipriano M. Major chemical constituents of Leucaena leucocephala and Salix babylonica leaf extracts. J Trop Agric 2011;49:95-8.  Back to cited text no. 43
    
44.
Dudonné S, Poupard P, Coutière P, Woillez M, Richard T, Mérillon JM, et al. Phenolic composition and antioxidant properties of poplar bud (Populus nigra) extract: Individual antioxidant contribution of phenolics and transcriptional effect on skin aging. J Agric Food Chem 2011;59:4527-36.  Back to cited text no. 44
    
45.
Van Der Klis JD, Biotechnik EV, Steyregg G. The potential of phytogenic feed additives in pigs and poultry. Europ Soc Veterinary Comparat Nutrit 2008;18:2-7.  Back to cited text no. 45
    
46.
Dieumou FE, Teguia A, Kuiate JR, Tamokou JD, Fonge NB, Dongmo MC. Influence des huiles essentielles du gingembre (Zingiber officinale) et d'ail (Allium sativum) sur les performances de croissance et la flore intestinale des poulets de chair. Livestock Res Rural Develop 2009;21.  Back to cited text no. 46
    
47.
Sethiya NK. Review on natural growth promoters available for improving gut health of poultry: An alternative to antibiotic growth promoters. Asian J Poult Sci 2016;10:1-29.  Back to cited text no. 47
    
48.
Bravo D, Pirgozliev V, Rose SP. A mixture of carvacrol, cinnamaldehyde, and capsicum oleoresin improves energy utilization and growth performance of broiler chickens fed maize-based diet. J Anim Sci 2014;92:1531-6.  Back to cited text no. 48
    
49.
Lai Y, Gallo RL. AMPed up immunity: How antimicrobial peptides have multiple roles in immune defense. Trends Immunol 2009;30:131-41.  Back to cited text no. 49
    
50.
Wang S, Zeng X, Yang Q, Qiao S. Antimicrobial peptides as potential alternatives to antibiotics in food animal industry. Int J Mol Sci 2016;17.  Back to cited text no. 50
    
51.
Messaoudi S, Kergourlay G, Dalgalarrondo M, Choiset Y, Ferchichi M, Prévost H, et al. Purification and characterization of a new bacteriocin active against Campylobacter produced by Lactobacillus salivarius SMXD51. Food Microbiol 2012;32:129-34.  Back to cited text no. 51
    
52.
Wang HT, Yu C, Hsieh YH, Chen SW, Chen BJ, Chen CY. Effects of albusin B (a bacteriocin) of Ruminococcus albus 7 expressed by yeast on growth performance and intestinal absorption of broiler chickens--Its potential role as an alternative to feed antibiotics. J Sci Food Agric 2011;91:2338-43.  Back to cited text no. 52
    
53.
Suresh G, Das RK, Kaur Brar S, Rouissi T, Avalos Ramirez A, Chorfi Y, et al. Alternatives to antibiotics in poultry feed: Molecular perspectives. Crit Rev Microbiol 2018;44:318-35.  Back to cited text no. 53
    
54.
Wen LF, He JG. Dose-response effects of an antimicrobial peptide, a cecropin hybrid, on growth performance, nutrient utilisation, bacterial counts in the digesta and intestinal morphology in broilers. Br J Nutr 2012;108:1756-63.  Back to cited text no. 54
    
55.
Liu T, She R, Wang K, Bao H, Zhang Y, Luo D, et al. Effects of rabbit Sacculus rotundus antimicrobial peptides on the intestinal mucosal immunity in chickens. Poult Sci 2008;87:250-4.  Back to cited text no. 55
    
56.
Allen HK, Trachsel J, Looft T, Casey TA. Finding alternatives to antibiotics. Ann N Y Acad Sci 2014;1323:91-100.  Back to cited text no. 56
    
57.
Huff WE, Huff GR, Rath NC, Balog JM, Donoghue AM. Alternatives to antibiotics: Utilization of bacteriophage to treat colibacillosis and prevent foodborne pathogens. Poult Sci 2005;84:655-9.  Back to cited text no. 57
    
58.
Johnson RP, Gyles CL, Huff WE, Ojha S, Huff GR, Rath NC, et al. Bacteriophages for prophylaxis and therapy in cattle, poultry and pigs. Anim Health Res Rev 2008;9:201-15.  Back to cited text no. 58
    
59.
Rodríguez-Rubio L, Martínez B, Donovan DM, Rodríguez A, García P. Bacteriophage virion-associated peptidoglycan hydrolases: Potential new enzybiotics. Crit Rev Microbiol 2013;39:427-34.  Back to cited text no. 59
    
60.
Schmelcher M, Donovan DM, Loessner MJ. Bacteriophage endolysins as novel antimicrobials. Future Microbiol 2012;7:1147-71.  Back to cited text no. 60
    
61.
Zhao XH, He X, Yang XF, Zhong XH. Effect of Portulaca oleracea extracts on growth performance and microbial populations in ceca of broilers. Poult Sci 2013;92:1343-7.  Back to cited text no. 61
    
62.
Kim SC, Kim JW, Kim JU, Kim IH. Effects of dietary supplementation of bacteriophage on growth performance, nutrient digestibility, blood profiles, carcass characteristics and fecal microflora in broilers. Korean J Poult Sci 2013;40:75-81.  Back to cited text no. 62
    
63.
Kittler S, Fischer S, Abdulmawjood A, Glünder G, Klein G. Effect of bacteriophage application on Campylobacter jejuni loads in commercial broiler flocks. Appl Environ Microbiol 2013;79:7525-33.  Back to cited text no. 63
    
64.
Bragg R, Van Der WW, Lee J, Coetsee E, Boucher C, Jansen A, et al. Infectious diseases and Nanomedicine I. Adv Experim Med Biol 2014;807:97-110.  Back to cited text no. 64
    
65.
Gadde U, Rathinam T, Lillehoj HS. Passive immunization with hyperimmune egg-yolk IgY as prophylaxis and therapy for poultry diseases--A review. Anim Health Res Rev 2015;16:163-76.  Back to cited text no. 65
    
66.
Xu Y, Li X, Jin L, Zhen Y, Lu Y, Li S, et al. Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: A review. Biotechnol Adv 2011;29:860-8.  Back to cited text no. 66
    
67.
Tamilzarasan K, Dinakaran A. Efficacy of egg yolk immunoglobulins (IGY) against enteric pathogens in poultry. Tamilnadu J 2009;5:264-8.  Back to cited text no. 67
    
68.
Cook ME. Antibodies: Alternatives to antibiotics in improving growth and feed efficiency. J Appl Poult Res 2004;13:106-19.  Back to cited text no. 68
    
69.
Mahdavi AH, Rahmani HR, Nili N, Samie AH, Soleimanian-Zad S, Jahanian R. Effects of dietary egg yolk antibody powder on growth performance, intestinal Escherichia coli colonization, and immunocompetence of challenged broiler chicks. Poult Sci 2010;89:484-94.  Back to cited text no. 69
    


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