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  Indian J Med Microbiol
 

Figure 4: Efficiency of Cas9/sgRNA mutagenesis. (a) Polymerase chain reaction amplification of the clustered regularly interspaced short palindromic repeat: CesA-like lines to generate a 300 bp product that could then be challenged with BssS1. (b) Polymerase chain reaction/ restriction digestion analysis demonstrates that all products can be cut with BssS1 indicating a lack of mutations in this sequence

Figure 4: Efficiency of Cas9/sgRNA mutagenesis. (a) Polymerase chain reaction amplification of the clustered regularly interspaced short palindromic repeat: <i>CesA</i>-like lines to generate a 300 bp product that could then be challenged with BssS1. (b) Polymerase chain reaction/ restriction digestion analysis demonstrates that all products can be cut with BssS1 indicating a lack of mutations in this sequence