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  Indian J Med Microbiol
 

Figure 2: Polymerase chain reaction products were amplified using the Cas9/NPTII-specific primers from Agrobacterium EHA105. The purified polymerase chain reaction products were separated by electrophoresis on a 2% (w/v) agarose gel in 0.5X TAE at 100 V for 30 minutes. Lane 1, 2 and 3 are polymerase chain reaction products of sgRNA constructs which were isolated from colonies of Agrobacterium EHA105, Lane 4: Hyperladder marker

Figure 2: Polymerase chain reaction products were amplified using the Cas9/NPTII-specific primers from <i>Agrobacterium</i> EHA105. The purified polymerase chain reaction products were separated by electrophoresis on a 2% (w/v) agarose gel in 0.5X TAE at 100 V for 30 minutes. Lane 1, 2 and 3 are polymerase chain reaction products of sgRNA constructs which were isolated from colonies of <i>Agrobacterium</i> EHA105, Lane 4: Hyperladder marker