• Users Online: 26
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2019  |  Volume : 3  |  Issue : 2  |  Page : 44-47

Mutagenesis of cellulose synthase (CesA-Like) Gene in tomato using clustered regularly interspaced short palindromic repeat/CAS9-system


Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

Correspondence Address:
Nurul Hidayah Samsulrizal
International Islamic University Malaysia
Malaysia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/MTSP.MTSP_11_19

Rights and Permissions

Background: During ripening, the changes in texture involved remodelling of cell walls of fruits including tomato and also alterations in tissue water relations caused by modifications in the cuticle. Aims and Objectives: To better understand the relationship between cell wall remodelling and fruit softening, an understanding of cell wall structure is necessary. Materials and Methods: Cellulose synthase gene that consists of cellulose synthase (CesA-like) plays important role in cellulose biosynthesis. However, CesA family genes are yet to be fully characterized in Solanaceae species. Results: In this study, we generated transgenic plants to test the role of CesA like gene in texture changes using tomato (Solanum lycopersicum) as a model system. We used the recently developed clustered regularly interspaced short palindromic repeat/Cas9 DNA editing technology to generate mutations in the target gene. Conclusion: Nevertheless, there was no mutation recovered in the CesA-like gene, and this indicates that this gene product is likely essential for regeneration of plantlets from tissue culture.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed240    
    Printed27    
    Emailed0    
    PDF Downloaded52    
    Comments [Add]    

Recommend this journal